In vitro evaluation of the antimicrobial activity of leaf extracts of Litsea iteodaphne against a selected group of bacteria including methicillin-resistant Staphylococcus aureus
In vitro evaluation of the antimicrobial activity of leaf extracts of Litsea iteodaphne against a selected group of bacteria including methicillin-resistant Staphylococcus aureus
Blog Article
Background: The quest for scientific endorsement of new drugs from plants continues due to the rising antibiotic resistance against pathogenic bacteria.Litsea iteodaphne is used in Sri Lanka in the Classes-Events treatment of infectious diseases.Therefore, in vitro antibacterial activity of L.iteodaphne plant extracts were evaluated against selected human pathogenic bacteria.
Materials and Methods: Antibacterial activity of 400, 40, and 4 mg/ml concentrations of hexane, ethanol, and aqueous leaf extracts of L.iteodaphne were evaluated against Staphylococcus aureus, Psedomona s aeruginosa, Klebsiella pneumoniae, Escherichia coli, and methicillin-resistant S.aureus (MRSA) clinical isolates using disc diffusion method.Minimum inhibitory concentration (MIC) was identified, and phytochemical screening was carried out.
Results: Significant zones of inhibition ranging from 5.7 mm to 8.1 mm, 7.1 mm to 8.
0 mm, and 7.6 mm were obtained for ethanol, hexane, and aqueous extracts at 400 mg/ml, respectively, against above four bacteria.For MRSA clinical isolates, zones of inhibition ranging from 6.1 mm to 10.
9 mm, 6.7 mm to 10.8 mm, and 6.4 mm Remote Starter with Interface Module to 8.
6 mm were obtained for ethanol, hexane, and aqueous extracts at 400 mg/ml, respectively.Ethanol extract of L.iteodaphne showed the lowest MIC value (0.0256 mg/ml).
Phytochemical screening revealed the presence of tannins, cardiac glycosides, reducing sugars, phenolic compounds, saponins, and flavonoids.Conclusions: L.iteodaphne crude leaf extracts showed promising antibacterial activity against Gram-positive and Gram-negative bacteria and clinical isolates of MRSA.Further investigations toward fractionation and the identification of an active compound will enhance the antimicrobial potential of L.
iteodaphne.